sumo protease 2 senp2  (BPS Bioscience)

Journal: Nature Communications

Article Title: Site-specific identification and quantitation of endogenous SUMO modifications under native conditions

doi: 10.1038/s41467-017-01271-3

Figure Lengend Snippet: Identification and quantitation of SUMO and Ub modification sites upon in vitro deSUMOylation in Hela cell extracts. a Extracts were either untreated or treated with varying amounts of SENP1 and SENP2 enzymes or Usp2cc and then analyzed by SDS-PAGE and blotted for SUMO1 or SUMO2/3 or ubiquitin. b Bar graph showing results from quantitation of KGG-peptides after treatment with SENP1/2, illustrating the reduction of KGG sites observed after WaLP digestion. Green bars indicate SUMO modification sites identified and quantified upon WaLP digestion (left, y axis) and blue bars indicate Ub modification sites identified and quantified upon trypsin digestion (right, y axis). c Bar graph showing results from quantitation of KGG-peptides after treatment with Usp2cc, illustrating the retention of KGG sites observed after WaLP digestion. Colors are the same as in b

Article Snippet: In vitro deSUMOylation reaction was performed by adding specific SUMO protease 1 (SENP1) and SUMO protease 2 (SENP2) (Life Sensors, Malvern, PA) at 10 units per milligram of lysate protein and incubating with lysate for overnight at room temperature.

Techniques: Quantitation Assay, Modification, In Vitro, SDS Page